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Clostridium difficile
Clostridium difficile
規格:
貨期:
編號:B81063
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Clostridium difficile
商品貨號 B81063
中文名稱(chēng) 艱難梭菌
模式菌株 模式菌株
其它保藏中心編號 ATCC9689
特征特性 Nucleotide (GenBank) : X94328 C.difficile spo0A gene. Nucleotide (GenBank) : AX110003 Sequence 736 from Patent WO0123604. Nucleotide (GenBank) : AX110007 Sequence 740 from Patent WO0123604. Nucleotide (GenBank) : AX110095 Sequence 828 from Patent WO0123604. Nucleotide (GenBank) : AX111484 Sequence 2217 from Patent WO0123604. Nucleotide (GenBank) : AX111505 Sequence 2238 from Patent WO0123604. Nucleotide (GenBank) : AX111525 Sequence 2258 from Patent WO0123604. Nucleotide (GenBank) : U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds. Nucleotide (GenBank) : S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt] Nucleotide (GenBank) : U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds. Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634 Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
具體用途 科研,生產(chǎn),產(chǎn)生細胞毒素,腸內研究
培養基 ATCC? Medium 2107: Modified Reinforced Clostridial ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
培養溫度 37 ℃, 厭氧氣體混合物,80% N2 -10% CO2 -10% H2
凍干物說(shuō)明 ·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養液或者無(wú)菌水溶解,接種在1-2個(gè)平板上,因凍干菌種處于休眠狀態(tài),請勿接種多個(gè)平板,以免因接種量不足而導致復蘇不成功;
保存說(shuō)明 1、長(cháng)期保存的儲存菌株以冷凍干燥保藏為主,特殊要求的菌種可用其它方法進(jìn)行保存,保存期可長(cháng)達幾年至幾十年。2、半儲存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主,在0℃以下保存,保存期為3-6個(gè)月。3、應用菌株以瓊脂斜面為主,在4℃保存,保存期為1-3個(gè)月。4、菌種的傳代不可超過(guò)6次。視菌種的特性及使用情況,一般儲存菌株每5年傳記代一次,每1-2年從儲存菌株轉接至半儲存菌株,半儲存菌株每3-6個(gè)月傳代一次,每1-3個(gè)月從半儲存菌株轉接接至應用菌株。
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