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Corneal Epithelial Cell Growth Kit
Corneal Epithelial Cell Growth Kit
規格:
貨期:
編號:B240695
品牌:Mingzhoubio

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    產(chǎn)品名稱(chēng) Corneal Epithelial Cell Growth Kit
    商品貨號 B240695
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
    Product Format frozen 1 kit
    Storage Conditions -20°C (or -70°C for long-term storage)
    Comments
    Corneal Epithelial Cell Growth Kit (ATCC PCS-700-040) contains components that when added to Corneal Epithelial Cell Basal Medium (ATCC PCS-700-030) creates a complete ATCC® Primary Cell Solution&trad; culture environment for epithelial cells derived from normal human corneal. The serum-free medium formulation is designed to support normal corneal epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.

    The final concentration for each component in ATCC complete corneal epithelial growth medium is as follows:
    • Apo-Transferrin: 5 µg/mL
    • Epinephrine: 1.0 µM
    • Extract P: 0.4%
    • Hydrocortisone: 100 ng/mL
    • L-Glutamine: 6 mM
    • rh Insulin: 5 µg/ml
    • CE Growth Factor: Proprietary formulation
    Complete Growth Medium
    1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight. 
    2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium. 
    3. Obtain one bottle of Corneal Epithelial Cell Basal Medium (485 mL) from cold storage.
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.

      6. Table 1. Corneal Epithelial Cell Growth Kit Components 

      Component                     

      Volume

      Volume
      Sterility Tests

      Final Concentration
      Functional Tests

      Apo-transferrin
      C of A

      0.5 mL
      梅經(jīng)理 17280875617 1438578920
      胡經(jīng)理 13345964880 2438244627
      周經(jīng)理 17757487661 1296385441
      于經(jīng)理 18067160830 2088210172
      沈經(jīng)理 19548299266 2662369050
      李經(jīng)理 13626845108 972239479
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