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pNRSac [p Sac]
pNRSac [p Sac]
規格:
貨期:
編號:B238982
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pNRSac [p Sac]
商品貨號 B238982
Designations pNRSac [p Sac]
Species Homo sapiens, human
Depositors RA Weinberg
Applications
The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.
Vector
Construct size (kb): 9.0
Insert
DNA: genomic
Insert lengths(kb): 7.0
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) [NRAS]
Alleles: A1, A2
Insert Size (kb) 7.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5, 3.5; PvuII--4.2, 3.4, 1.0, 0.6.
The insert can transform NIH 3T3 cells. The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.
Contains pBR322 (EcoRI/PvuII, 2.05 kb); SV40 ori (PvuII/HindIII, 344 bp); exon 1 (HindIII/HindIII); exon 2 (HindIII/EcoRI, 760 bp); exon 3/exon 4 (EcoRI/SacI, 5 kb), and SV40 polyadenylation signal (SacI/EcoRI).
References

Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594

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