收集最新中文国产中文字幕_pBSL121_ATCC代理_寧波明舟生物科技有限公司，細胞，菌株，ATCC，保藏中心

pBSL121

規格:

貨期:

編號:B235040

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

產(chǎn)品名稱(chēng)	pBSL121
商品貨號	B235040
Designations	pBSL121
Depositors	MF Alexeyev
Biosafety Level	1
Vector Information	Size (kb): 4.0510001182556150 Vector: pBSL121 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,cmlR Construct size (kb): 4.051000118255615 Features: marker(s): ampR promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: cat
Applications	contains easily purifiable cassette(s) for construction chloramphenicol acetyltransferase
Comments	Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.0; EcoRI--2.9, 1.2; BamHI--2.9, 1.2. Contains a chloramphenicol acetyltransferase (cat) cassette flanked by multiple cloning sites in tandem orientation. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. The EcoRI site was removed.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
Shipped	freeze-dried

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