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AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]
AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]
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貨期:
編號:B233568
品牌:Mingzhoubio

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產(chǎn)品名稱(chēng) AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]
商品貨號 B233568
Designations AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]
Species Homo sapiens, human
Depositors A Gnirke
Vector
Construct size (kb): 551.0
Insert
DNA: genomic
Insert lengths(kb): 540.0
Tissue: lymphoblastoid CGM1 cell line
Gene product: interferon, gamma transducer 1(interferon, gamma receptor accessory factor) [GART]
Insert Size (kb) 540.0
Media ATCC® Medium 1798: aAHC medium for YAC clones
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Consists of 5 SfiI fragments, 3 internal ones (70, 110, and 300 kb) and the YAC ends (50 and 70 kb).
The smallest fragment found to contain all the exons for GART is a 45 kb BssHII fragment.
The GART gene is toward the acentric end of the clone and the IFNGT1 gene is within 150 kb of the centric end.
When transferred into CHO cells, the clone complements the purine requirement of CHO Ade-C cells (lacking PRGS/GARS) and Ade-CG cells (lacking PRGS/GARS and PAIS/AIRS).
Both ends of the insert map to chromosome 21, suggesting a non-chimeric insert in this YAC.
References

Gnirke A, et al. Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes. EMBO J. 10: 1629-1634, 1991. PubMed: 2050105

Gnirke A, Huxley C. Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNA. Somatic Cell Mol. Genet. 17: 573-580, 1991. PubMed: 1767336

Cook JR, et al. Yeast artificial chromosome fragmentation vectors that utilize URA3 selection. Genet. Anal. Tech. Appl. 10: 109-112, 1993. PubMed: 8204303

Cook JR, et al. Sublocalization of the human interferon-gamma receptor accessory factor gene and characterization of accessory factor activity by yeast artificial chromosome fragmentation. J. Biol. Chem. 269: 7013-7018, 1994. PubMed: 8120065

Soh J, et al. Identification of a yeast artificial chromosome clone encoding an accessory factor for the human interferon gamma receptor: evidence for multiple accessory factors. Proc. Natl. Acad. Sci. USA 90: 8737-8741, 1993. PubMed: 8378357

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