| 產(chǎn)品名稱(chēng) |
ME 1 |
| 商品貨號 |
B165134 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27. The antibody cross-reacts with HLA B14 and HLA Bw46. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27 |
| Comments |
Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with HLA B14 and HLA Bw46. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.016 mM thymidine 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5%. |
| Isotype |
IgG1 |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ellis SA, et al. Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum. Immunol. 5: 49-59, 1982. PubMed: 6981636
|
