| 產(chǎn)品名稱(chēng) |
MD2 |
| 商品貨號 |
B165117 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
In the mouse thymus, MD2 is specific for dendritic cells in the medulla. The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies. It also reacts with epithelial elements in a variety of mouse tissues. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium |
| Comments |
Animals were immunized with mouse thymic stromal tissue. Spleen cells were fused with Sp2/0 myeloma cells. In the mouse thymus, MD2 is specific for dendritic cells in the medulla. The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies. It also reacts with epithelial elements in a variety of mouse tissues. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with non-essential amino acids, 1 mM sodium
pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
RV Rouse |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413
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