| 產(chǎn)品名稱(chēng) |
M3WT8 |
| 商品貨號 |
B165054 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Applications |
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor. |
| Clinical Data |
female |
| Receptor Expression |
acetylcholine, muscarinic m3 |
| Comments |
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor. The resulting cells were co-transfected with pMSVNeo and selected for growth in medium containing 0.5 mg/ml G418. M3WT8 cells are reported to express 75 fmol of receptor protein per mg of membrane protein. |
| Complete Growth Medium |
Ham's F12 medium with 0.05 to 0.1 mg/ml G418, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37C) until the cells detach (usually 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: At the time of subcultivation |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
CM Fraser |
| Deposited As |
Cricetulus griseus |
| References |
Buck MA, Fraser CM. Muscarinic acetylcholine receptor subtypes which selectively couple to phospholipase C: pharmacological and biochemical properties. Biochem. Biophys. Res. Commun. 173: 666-672, 1990. PubMed: 2175603
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