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M1
M1
規格:
貨期:
編號:B165032
品牌:Mingzhoubio

標準菌株
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產(chǎn)品名稱(chēng) M1
商品貨號 B165032
Organism Mus musculus, mouse
Cell Type myeloblast
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease myeloid leukemia
Strain SL
Applications
Can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS and WEHI-3 conditioned medium.
Derivation
Established in vitro from spontaneous myeloid leukemia of SL strain mice.
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Name of Depositor P Ralph
Deposited As Mus musculus
References

Ralph P, et al. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J. Immunol. 130: 108-114, 1983. PubMed: 6847876

Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J. Cell. Physiol. 74: 223-234, 1969. PubMed: 4187945

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022

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