| 產(chǎn)品名稱(chēng) |
K204 |
| 商品貨號 |
B164899 |
| Organism |
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with mouse non k haplotype class I major histocompatibility antigens (H-2). |
| Derivation |
Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against H-2 from non k haplotype mice |
| Cellular Products |
immunoglobulin; monoclonal antibody; against H-2 from non k haplotype mice |
| Comments |
Animals were immunized with 3T3-Swiss albino cells (ATCC CCL-92). Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. The antibody reacts with mouse non k haplotype class I major histocompatibility antigens (H-2). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 0.05 mM 2-mercaptoethanol and 4 mM
L-glutamine, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
M Mage, K Yamada, T Hasegawa |
| Deposited As |
rat (B cell); rat (myeloma) |
| References |
Nardelli B, et al. Polyacrylamide-streptavidin: A novel reagent for simplified construction of soluble multivalent macromolecular conjugates. J. Immunol. Methods 120: 233-239, 1989. PubMed: 2738413
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