| 產(chǎn)品名稱(chēng) |
Hybridoma 231 |
| 商品貨號 |
B164810 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Comments |
Animals were immunized with canine lymphoma cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Cryopreservation |
Complete growth medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
Wistar Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992
|
