| 產(chǎn)品名稱(chēng) |
ES-E14TG2a |
| 商品貨號 |
B164419 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Cell Type |
embryonic stem cell , blastocyst derived |
| Product Format |
frozen |
| Morphology |
spherical |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
embryo, blastocyst embryo, blastocyst |
| Strain |
129/Ola |
| Applications |
This cell line is a suitable transfection host.
They may also be of use in studies of early post implantation structures. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987. |
| Comments |
The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.
The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).
The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
When injected into blastocysts, the cells can colonize the germline.
After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated mitomycin-C treated feeder cells (ATCC 56-X.2).
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
T Doetschman |
| Deposited As |
Mus musculus |
| References |
Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655
Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599
Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574
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