| 產(chǎn)品名稱(chēng) |
CCD-944Sk |
| 商品貨號 |
B164213 |
| Organism |
Homo sapiens, human |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
66 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
The line was established from skin taken from the abdomen. |
| Derivation |
The line was established from skin taken from the abdomen. |
| Clinical Data |
female Caucasian 66 years |
| Comments |
The line was established from skin taken from the abdomen. Cells from the current passage (2) have experienced about 14 population doublings beyond the biopsy material Subculture every 6 to 8 days. |
| Complete Growth Medium |
Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate; 90%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 12 D5S818: 10,11 D7S820: 7,10 THO1: 9,9.3 TPOX: 8 vWA: 16,18 |
| Passage History |
Cells from the current passage (2) have experienced about 14 population doublings beyond the biopsy material Subculture every 6 to 8 days. |
