| 產(chǎn)品名稱(chēng) |
BL-3 |
| 商品貨號 |
B164035 |
| Organism |
Bos taurus, cow |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphosarcoma; leukemia |
| Age |
3 months |
| Gender |
male |
| Strain |
Hereford |
| Applications |
The cells do not produce bovine leukemia virus. They are of possible use in making vaccines against bovine leukemia virus. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data |
male |
| Comments |
The cells do not produce bovine leukemia virus. They are of possible use in making vaccines against bovine leukemia virus. Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
Regents of the University of California |
| Deposited As |
Bos taurus |
| U.S. Patent Number |
|
| References |
Theilen GH, et al. Bovine leukemia: establishment and morphologic characterization of continuous cell suspension culture, BL-1. J. Natl. Cancer Inst. 40: 737-749, 1968. PubMed: 4171552
Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
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