| 產(chǎn)品名稱(chēng) |
BB |
| 商品貨號 |
B163998 |
| Organism |
Ictalurus nebulosus, bullhead, brown |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
2 years |
| Virus Resistance |
herpes simplex; poliovirus 2; vaccinia |
| Comments |
BB cells will grow at 4 to 34C with an optimum of between 25 and 30C. The cells do not do well when cultured on a glass surface. |
| Complete Growth Medium |
Minimum essential medium (Eagle) with 2mM L-glutamine and Hanks' BSS with 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions |
Temperature: 23.0°C |
| Name of Depositor |
RG Malsberger |
| Deposited As |
Ictalurus nebulosus |
| References |
Wolf K, Quimby MC. Fish cell and tissue culture In: Wolf K, Quimby MC. Fish physiology, 3 New York Academic Press pp. 253-301, 1969
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