| 產(chǎn)品名稱(chēng) |
B21-2 |
| 商品貨號 |
B163977 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8-U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A b,d |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A b,d |
| Tumorigenic |
Yes |
| Effects |
Yes, in irradiated (600R) pristane primed BALB/c mice |
| Comments |
Animals were immunized intravenously with live dendritic cells from BALB/c mice. Spleen cells were fused with P3X63Ag8-U1 myeloma cells. The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
RM Steinman |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Steinman RM, et al. Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J. Exp. Med. 152: 1248-1261, 1980. PubMed: 6448907
Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426
Schuler G, Steinman RM. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161: 526-546, 1985. PubMed: 3871837
Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
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