| 產(chǎn)品名稱(chēng) |
Anti-My-10 clone 28/8/8/14/4 |
| 商品貨號 |
B163946 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells). |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells |
| Comments |
Animals were immunized with KG-1a cells (see ATCC CCL-246.1). Spleen cells were fused with myeloma cells. The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells). |
| Complete Growth Medium |
RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Johns Hopkins School of Medicine |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Civin CI. Human stem cells. US Patent 4,714,680 dated Dec 22 1987
Civin CI, et al. My-1, new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood 57: 842-845, 1981. PubMed: 6163493
Page PL, et al. Cytotoxic reduction and regeneration of murine marrow stem cells. Exp. Hematol. 11: 202-211, 1983. PubMed: 6832246
|
