| 產(chǎn)品名稱(chēng) |
2E8 |
| 商品貨號 |
B163684 |
| Organism |
Mus musculus, mouse |
| Tissue |
bone marrow |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
immature |
| Strain |
BALB/c.xid |
| Applications |
The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Antigen Expression |
Antigen expression: H-2 d; Ia d; BP-1 |
| Receptor Expression |
interleukin 7 (IL-7) |
| Genes Expressed |
immunoglobulin (surface IgM) |
| Cellular Products |
immunoglobulin (surface IgM) |
| Comments |
The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serum
|
| Subculturing |
Every 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture.
Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7.
Medium Renewal: Every 2 to 4 days |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Isotype |
IgM , IgM |
| Name of Depositor |
PW Kincade |
| Deposited As |
Mus musculus |
| References |
Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182
Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. PubMed: 1821694
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