| 產(chǎn)品名稱(chēng) |
187.1 |
| 商品貨號 |
B163608 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
mixed suspension-adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse kappa light chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse kappa light chain |
| Comments |
Animals were immunized with MPC-11 immunoglobulin. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Subculture by scraping attached cells and transferring with floating cells to a new flask. Start cultures at 2 X 10 (5) cells/ml and maintain between 1 X 10 (5) and 1 X 10 (6) cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5%
|
| Isotype |
IgG1 |
| Name of Depositor |
MD Scharff |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620
Yelton DE, et al. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma 1: 5-11, 1981. PubMed: 6821395
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