| 產(chǎn)品名稱(chēng) |
Adeno-associated virus 5 |
| 商品貨號 |
B163263 |
| Classification |
Parvoviridae, Dependovirus, Adeno-associated virus 5 |
| Agent |
Adeno-associated virus 5 |
| Strain |
Original |
| Applications |
Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
clinical specimen - human |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Comments |
Depends upon adenovirus for replication. Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells. *Helper virus may be inactivated by heating to 56C for 30 min. |
| Effect on Host |
in vitro effects: Adeno-associated virus shows no independent cytopathic effects Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE. Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? LLC-MK2 (ATCC? CCL-7.1TM) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 4 - 5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). [Note: requires viral helper for productive infections, e.g., VR-846] |
| Cryopreservation |
Storage temperature: -70°C |
| Name of Depositor |
U Bantel-Schaal, H zur Hausen, U Bantel-Schaal |
| Special Collection |
NCRR Contract |
| Source |
clinical specimen - human |
| References |
or colder
Georg-Fries B, et al. Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. Virology 134: 64-71, 1984. PubMed: 6200995
Bantel-Schaal U, zur Hausen H. Characterization of the DNA of a defective human parvovirus isolated from a genital site. Virology 134: 52-63, 1984. PubMed: 6324476
can also be grown on other human and rodent cell lines
penile flat condylomatous lesion
requires viral helper for productive infections
confirm growth with PCR
Bantel-Schaal U, et al. Human adeno-associated virus type 5 is only distantly related to other known primate helper-dependent parvoviruses. J. Virol. 73: 939-947, 1999. PubMed: 9882294
Bantel-Schaal U, et al. Endocytosis of adeno-associated virus type 5 leads to accumulation of virus particles in the Golgi compartment. J. Virol. 76: 2340-2349, 2002. PubMed: 11836412
or colder
|
| Cross References |
Nucleotide (GenBank) :
AF085716
Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes, complete cds.
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