| 產(chǎn)品名稱(chēng) |
Murine hepatitis virus |
| 商品貨號 |
B162856 |
| Classification |
Nidovirales, Coronaviridae, Coronavirus,Group 2 |
| Agent |
Murine hepatitis virus |
| Strain |
MHV-S Log #3239/17 |
| Common Name |
Murine hepatitis virus |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Highly infectious in susceptible mouse colonies. Despite high mortality, the clinical signs in infected mice are often non-specific, with weakness or are suggestive of encephalitis with spasticity. Necropsy occasionally reveals small yellow or whitish spots on the liver. Highly infectious in susceptible mouse colonies. |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects in tissue culture CPE, apoptosis and slough off in clumps |
| Recommended Host |
NCTC clone 1469 cells (ATCC CCL-9.1) Recommended Host: NCTC clone 1469 cells (ATCC? CCL-9.1™) newborn mouse; NCTC clone 1469 cells (ATCC CCL-9.1) |
| Growth Conditions |
Temperature: 37°C
Incubation: 18 to 48 hours at 37°C, a 5% CO2 in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). |
| Name of Depositor |
MJ Collins |
| Special Collection |
ATCC |
| Source |
Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice |
| References |
Hartley JW, et al. Mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161-165, 1963. PubMed: 13982823
|
