| 產(chǎn)品名稱(chēng) | Human echovirus 2 |
|---|---|
| 商品貨號 | B162835 |
| Classification | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As | Echovirus 2 |
| Agent | Human echovirus 2 |
| Strain | Cornelis |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | Derived by adaptation of primary cell culture product ATCC® VR-1039™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1867™ and ATCC® VR-1039™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
| Effect on Host | CPE, refractile rounding and sloughing |
| Recommended Host | Vero cells (ATCC® CCL-81™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 16-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | NIH/NIAID |
| Source | Feces from a patient with aseptic meningitis, Connecticut, 1951. |
| References | Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
