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XHJ-12.4
XHJ-12.4
規格:
貨期:
編號:B161997
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) XHJ-12.4
商品貨號 B161997
Designations XHJ-12.4
GenBank Number

J04509

Species Mus musculus, mouse
Depositors D Nathans
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.669999957084656
Tissue: activated 3T3 cell line
Gene product: Jun-D oncogene( Jun protooncogene related gene d1, Jun-D protooncogene) [Jund]
Insert Size (kb) 1.670
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; HindIII--4.7; BamHI--4.7; PstI--4.7; SacI--4.7; AccI--3.6, 1.0.
jun-D appears to be expressed at a higher constitutive level than c-jun or jun-B, and is activated only slightly by serum growth factors.
The insert contains the following unique sites (in bp from the T7 promoter side [5']): BstEII--446; SfiI--572; AccI--654; FspI--916; HincII--1083; DraI--1487.
Although there is no characteristic poly(A) signal, there is an AGTAAA sequence near the 3' end. There is also a single ATTTA element, multiple copies of which are associated with mRNA instability.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Ryder K, et al. Jun-D: A third member of the Jun gene family. Proc. Natl. Acad. Sci. USA 86: 1500-1503, 1989. PubMed: 2493644

Howard TA, et al. A linkage map of mouse chromosome 8: further definition of homologous linkage relationships between mouse chromosome 8 and human chromosomes 8, 16, and 19. Genomics 10: 207-213, 1991. PubMed: 1675193

Daniel Nathans, personal communication

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